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anti sc tpa  (Innovative Research Inc)


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    Innovative Research Inc anti sc tpa
    Anti Sc Tpa, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti sc tpa/product/Innovative Research Inc
    Average 93 stars, based on 3 article reviews
    anti sc tpa - by Bioz Stars, 2026-03
    93/100 stars

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    FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from <t>CHO-tPA</t> transfectant pool. B : detection of secreted tPA using an anti-tPA <t>antibody</t> <t>conjugated</t> to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).
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    FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from <t>CHO-tPA</t> transfectant pool. B : detection of secreted tPA using an anti-tPA <t>antibody</t> <t>conjugated</t> to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).
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    FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from <t>CHO-tPA</t> transfectant pool. B : detection of secreted tPA using an anti-tPA <t>antibody</t> <t>conjugated</t> to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).
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    FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from <t>CHO-tPA</t> transfectant pool. B : detection of secreted tPA using an anti-tPA <t>antibody</t> <t>conjugated</t> to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).
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    FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from <t>CHO-tPA</t> transfectant pool. B : detection of secreted tPA using an anti-tPA <t>antibody</t> <t>conjugated</t> to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).
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    FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from <t>CHO-tPA</t> transfectant pool. B : detection of secreted tPA using an anti-tPA <t>antibody</t> <t>conjugated</t> to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).
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    FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from <t>CHO-tPA</t> transfectant pool. B : detection of secreted tPA using an anti-tPA <t>antibody</t> <t>conjugated</t> to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).
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    FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from CHO-tPA transfectant pool. B : detection of secreted tPA using an anti-tPA antibody conjugated to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).

    Journal: BMC Biotechnology

    Article Title: Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins

    doi: 10.1186/1472-6750-9-42

    Figure Lengend Snippet: FLSSM applied to different products and cell lines . A , A' and C : transmitted light images. A and B : colonies from CHO-tPA transfectant pool. B : detection of secreted tPA using an anti-tPA antibody conjugated to Alexa488 in semi-solid medium. Note the presence of positive and negative colonies in the same field. Arrow indicates positive, high-producing clone; A ': insert blown up from A showing that a precipitate around the positive colony is visible in transmitted light microscopy. C and D : colony from 293-IGF-E5 transfectant pool. D : detection of secreted IGF-E5 (small fluorescent dots at the colony periphery using an anti-His Tag antibody conjugated to FITC in semi-solid medium).

    Article Snippet: IGF-E5 blots were treated with an anti-His Tag antibody (Serotec) followed by an anti-mouse conjugated to Alexa488 (Invitrogen). tPA blots were treated with a sheep anti-tPA primary antibody (Cedarlane) followed by a rabbit anti-sheep antibody conjugated to Cy3 (Jackson, Immunoresearch Labs, West Grove, PA).

    Techniques: Transfection, Light Microscopy